Response Surface Methodology for the Optimized Production of an Alkalophilic Laccase from Γ-proteobacterium Jb
نویسندگان
چکیده
γ-Proteobacterium JB, an alkali-tolerant soil isolate, produced laccase (8X10 nkat/L) in M162 medium. The optimization of process conditions (pH, incubation time, agitation, and CuSO4 concentration) for laccase production during submerged fermentation was carried out using response surface methodology (RSM) based on a central composite design (CCD). Maximum laccase production achieved was 7.4 X 10 nkat/L at pH 8.0, 210 rpm, 100 μM of CuSO4 after 60 h of incubation. This design of experiment methodology increased laccase production by 9.3 fold over the control. Experimental findings were in close agreement with the model predictions.
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